Effects of KMUP-1 on mRNA expression of osteoclastogenesis-related genes.

<p>Total RNA was extracted from RAW264.7 cells cultured for 24 h in the presence of RANKL (10 ng/ml) and KMUP-1 (0–10 µM), or with pretreatment of PKA inhibitor H89 (10 µM) and PKG inhibitor KT5823 (3 µM) for 30 min. The mRNA expression of the indicated genes was analyzed by real time RT-PCR. Each value represents the mean ± S.E.M. of three independent experiments, with triplicate determinations in each experiment. ^##<i>P</i><0.01 compared with control; *<i>P</i><0.05 compared with RANKL alone; ⁺<i>P</i><0.05 compared with KMUP-1 10 µM plus RANKL.