p38 MAP Kinase activation within immortalized urothelial cells following tryptase stimulation.

<p>Upper panel: Representative immunoblots of p38 MAP kinase measured as ATF-2 phosphorylation in tryptase-stimulated (20 ng/ml) human urothelial cells (HUC) and immortalized cells from normal and IC/PBS patients. Lower panel: p38 MAP kinase activity was significantly increased in tryptase-stimulated HUC (X) and immortalized cells from normal (filled circles) and IC/PBS bladders (filled squares). p38 MAP kinase activity is expressed as a fold increase over unstimulated values and was determined from 25 µg cytosolic protein. Data shown are mean±SEM for results from 4 different experiments using cell isolations from 3 (HUC) donors or 4 separate patients (immortalized cells from normal and IC/PBS bladder).