Hydrogen peroxide prompts lagging ERK and p38 activation in AD fibroblasts.

<p>PS-1 (M146L) and non-PS AD and normal control human skin fibroblasts were treated with 250 µM H₂O₂ for 0–60 min and immunoblotted for ERK and p38 as described in previous figures. ERK activation was digitally analyzed by Odyssey (A). ERK fold activation was quantified as a percentage of basal activation in buffer treated cells and normalized to Tubulin as loading control (B). Phospho-p38 levels were analyzed by ECL (C) and p38 activation was quantified as O.D. units normalized to tubulin as loading control (D). All graphs show mean±S.E. Statistical analysis was performed via t-test with *p-value<0.05 and **p<0.005; n = 4.