EGFP-tagged PrP constructs used in this study.

<p>The structural domains of zebrafish (zf) PrP-1, PrP-2 and mouse (m) PrP are represented as follows: leader peptide containing the polybasic motif (L) in violet, repetitive domain (Rep) in blue, hydrophobic region (Hyd) in red, globular domain (Glob) in light blue and GPI-anchored signal (GPI) in yellow. Amino acid (aa) positions of mouse and fish PrP domains are indicated. The EGFP fluorescence tags are depicted as green triangles. Deletion constructs lacking Rep (ΔRep), Hyd (ΔHyd), Glob (ΔGlob), Rep+Hyd+Glob (ΔCore), GPI (GPI⁻) and N-glycosylation sites (Glyc⁻) are shown for mouse PrP only. PrP domains were defined by evolutionary criteria <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0070327#pone.0070327-RiveraMilla1" target="_blank">[13]</a>.