FACS analysis of EGFP⁺ cells isolated from the cortex of 20-day-old mice.

<p>EGFP⁺ cells sorted based on EGFP intensity (x axis) and side scatter intensity reflecting cell size (y axis). During the selection of cells by FACS, their position is automatically saved in the plot, and thus it is possible to determine the expression profile of each individual cell in the plot after qPCR analysis. The red line indicates the sorter gate and each dot represents one cell. Cells positive for <i>Cspg4</i> and/or <i>Pdgfra</i> are indicated in green while those positive only for <i>Eaat1</i> and/or <i>Glul</i> are shown in red. The latter are also drawn larger for better resolution. The small black dots represent all of the cells from which we selected our samples.