Isolation of NCCs from <i>P0-Cre/Floxed-EGFP</i> mouse embryos by fluorescence-activated cell sorting.

<p>(A) Craniofacial and trunk regions were indicated in the whole body by observation of direct EGFP fluorescence in E12.5 mice. (B) Representative EGFP-gated flow cytometric analysis charts clearly showed two populations, EGFP positive and negative at all examined embryonic ages. (C) The ratio of collected EGFP⁺ cells showed a significantly higher frequency in the craniofacial region than that in the trunk region of all examined embryonic ages. Results were evaluated using the Student's <i>t</i>-test. (mean ± SD, n = 5 per group, **p<0.005).