Analysis of hGFAP:CKO mice using the Aldh1l1-EGFP transgene shows presence of dysregulated astrocytes with increase EGFP signals.

<p>(a and b) P16 hGFAP:CKO forebrains have decreased sizes. (c,d, and e) P16 cortex and (f, g, and h) spinal cord sections indicate the presence of Aldh1l1-EGFP positive cells with higher EGFP signal in the hGFAP:CKO CNS. (i) The increase in EGFP signals can be visualized using FACS (blue represents P16 wildtype and dotted black represents P16 hGFAP:CKO Aldh1l1-EGFP positive cells). X axis in i represents EGFP levels in log2 scale. Y axis represents cell numbers. Scale bars in a and b represent 5 mm. Scale bars in c, d, and e represent 50 μm. Scale bars in f, g, and h represent 100 μm. FACS data for panel i is representative of 3 experiments. All histological experiments are representative data based on 3 independent observations.