Effects of MyD88 small interference (si) RNA and MAPK inhibitors on translocation of GATA-2.

<p>RAW 264.7 cells were exposed to lipopolysaccharide (LPS), MyD88 siRNA, and a combination of MyD88 siRNA and LPS. Amounts of GATA-2 were immunodetected (A, <i>top panel</i>). PCNA was measured as the internal control (<i>bottom panel</i>). These protein bands were quantified and statistically analyzed (B). RAW 264.7 cells were pretreated with 10 µM MAPK inhibitors, including SB203580 (SB), SP600125 (SP), and PD98059 (PD), for 1 h and then exposed to LPS. Nuclear GATA-2 was immunodetected (C, <i>top panel</i>). Amounts of PCNA were measured as the internal control (<i>bottom panel</i>). These protein bands were quantified and statistically analyzed (D). The immunoblotting results shown are a representative of 6 experiments, and the other statistically analyzed results are a compilation of 6 replications. Each value represents the mean ± SD. An asterisk (*) and pound sign (^#) respectively indicate that the value significantly (<i>p</i> < 0.05) differed from the respective control and LPS-treated group.