Effects of lipopolysaccharide (LPS) on the translocation and transactivation of GATA-2.

<p>RAW 264.7 cells were exposed to 100 ng/ml LPS for 1, 3, and 6 h. Amounts of nuclear GATA-2 were immunodetected (A, <i>top panel</i>). Levels of nuclear PCNA were measured as the internal control. These immunorelated proteins were quantified and statistically analyzed (<i>bottom panel</i>). RAW 264.7 cells were treated with 100 ng/ml LPS, the translocation of GATA-2 from the cytoplasm to nuclei were analyzed using confocal microscopy (B). The transactivation activity of GATA-2 was assayed using an EMSA analysis (C). These DNA-protein bands were quantified and statistically analyzed (D). The immunoblotting, confocal, and DNA-protein binding results shown are a representative of at least 3 experiments, and the other statistically analyzed results are a compilation of 6 replications. Each value represents the mean ± SD. An asterisk (*) indicates that the value significantly differed from the respective control, <i>p</i> < 0.05.