Roles of MyD88 in lipopolysaccharide (LPS)-induced activation of MEK1/2.

<p>RAW 264.7 cells were subjected to MyD88 small interfering (si) RNA for 24 and 48 h. Levels of MyD88 were immunodetected (A, <i>top panel</i>). β-Actin was measured as the internal control (<i>bottom panel</i>). These protein bands were quantified and statistically analyzed (B). RAW 264.7 cells were exposed to LPS, MyD88 siRNA, and a combination of MyD88 siRNA and LPS. Amounts of phosphorated MEK1/2 were immunodetected (C, <i>top panel</i>). MEK1 was measured as the internal control (<i>bottom panel</i>). These protein bands were quantified and statistically analyzed (D). The immunoblotting results shown are a representative of 6 experiments, and the other statistically analyzed results are a compilation of 6 replications. Each value represents the mean ± SD. An asterisk (*) and pound sign (#) respectively indicate that the value significantly (<i>p</i> < 0.05) differed from the respective control and LPS-treated group.