Shh induced HDAC activation and proliferation in CGP cells.

<p>A, Phase contrast image of CGP cells incubated with DMSO, 0.1 µM SAG or 1 µM SAG for 6 days. Scale bar represents 100 µm. B, HDAC activity was measured from CGP cells incubated either with DMSO or different concentrations of SAG for 2, 4 and 6 days. Bars correspond to mean ± S.E. C, Phase contrast images of CGP cells from Smo/Smo mice after 1,3,5 and 7 days in vitro culture. Scale bar represents 100 µm. D, HDAC activity was measured from cultured CGP cells isolated from wild type and Smo/Smo mice. Bars correspond to mean ± S.E.