Inhibition of HDAC activity by TSA affects mice myeloid differentiation, favoring progenitors cells (R4) after 24 and 48 hours of culture.

<p>(A-D) After 24 h of culture, myeloid cells were analyzed with the phenotypic markers CD11b and Gr1. (E) Percentage of cells in the regions: R2 (Gr1^highCD11b^low), R3 (Gr1^highCD11b^high) R4 (Gr1^lowCD11b^low) and R5 (Gr1^lowCD11b^high) (n = 4 mice per group). (F-I) After 48 h of culture, myeloid cells were analyzed with the phenotypic markers CD11b and Gr1. (J) Percentage of cells in R2, R3, R4 and R5 regions. Data are means ± SD. Statistically significant differences, * p <0.05 by two-way ANOVA for repeated measurements followed by the Sidak test for correction of the p value.