CAV1 silencing reduces vascularization in EWS.

<p><b>A.</b> Immunoblot showing substantially reduced CAV1 levels in selected clones of RDES, TC71 and SKES1 cells stably expressing a CAV1 shRNA compared with untransfected cells and cells transfected with an empty vector. β-Actin was used as loading control. <b>B.</b> Mice xenograft volumes comparing tumors from grouped control cells (parental n = 5; empty vector n = 5) and 2 CAV1 knocked-down cells (n = 5 for every clone); bars, SD (*P≤0.05) <b>C.</b> Hematoxylin and Eosin (H&E) staining from corresponding paraffin-embedded tumors. Asterisks indicate necrotic lakes Scale bar, 50 µm. <b>D.</b> CD31 immunohistochemistry detecting positive blood vessels in paraffin-embedded tumors. Arrows indicate blood vessel staining Scale bar, 50 µm. Graph shows MVD comparing all three models and showing the constant reduction of blood vessels in CAV1 knocked-down cells; bars, SD (*P≤0.05).