CAV1 silencing results on reduced endothelial cell migration.

<p><b>A.</b> Quantification of endothelial cell growth comparing conditioned media (CM) from CAV1 knocked-down models versus RPMI without serum as negative control (n = 3). <b>B.</b> Quantification of endothelial cell migration where CM from RDES and TC71 models was used as chemoattractant for endothelial cells; bars, SD (*P≤0.05 n = 3) <b>C.</b> Phospho-FAK immunofluorescence. Endothelial cells cultured with CM from TC71 CAV1 knocked-down model, showing a decrease on phospho-FAK sprouts. Scale bar, 20 µm. <b>D.</b> FAK expression was analyzed in PAEC cells grown in CM from the TC71 model. Blot shows higher expression of 35 kd cleaved FAK form in cells grown with TC71 control CM than with TC71 Sh CM.