The interaction of HPat and GW182 protein.

<p>Immunoprecipitation analysis of <i>Drosophila</i> S2 cell lysates using anti-HPat (<b>A</b>) or anti-GW182 (<b>B, C</b>) antibodies or preimmune sera. Input (lane 1) and immunoprecipitates (lanes 2, 3) were separated on SDS-PAGE and analyzed by Western blot analysis using anti-HPat, anti-GW182 or anti-AGO1 antibody. In A) and B) 1.5% of the input (total clarified cell lysate) and 40% of the immunoprecipitate were separated on a SDS-PAGE, while in C) 2.5% of the input and only 10% of the immunoprecipitate were separated. The asterix indicates cross-reactivity of the secondary antibody with the immunoglobulin heavy chain of the antibody used for immunoprecipitation.