Interaction of OCILRP2 and DAP12 in GST pull-down and co-immunoprecipitation assays.

<p>The GST pull-down assay was carried out using purified beads that contained GST, GST-DAP12, or GST-OCILRP2. Precipitated OCILRP2 or DAP12 was detected by western blotting using an anti-OCILRP2 or anti-DAP12 antibody, respectively (a, b). 293T cells were grown in 6-cm dishes and transfected with the pCDNA3-HA-OCILRP2 and pDsRed-C1-DAP12 plasmids, respectively. OCILRP2 and DAP12 were detected by western blotting using an anti-HA antibody or an anti-DAP12 antibody (c). Schematic diagram of OCILRP2 predicted by SMART software. The green column represents the transmembrane region (amino acids 57–80) (d). The GST pull-down assay was carried out using purified beads that contained GST, full-length GST-OCILRP2, GST-OCILRP2i (aa 1–57), or GST-OCILRP2e (aa 80–221). Precipitated DAP12 was detected by western blotting using an anti-DAP12 antibody (e).