The cGMP analogue, 8-Br-cGMP, promotes NSC neuronal differentiation through CNG channel activation.

<p>(<b>A</b>) Summary bar graph showing an increased number of MAP2⁺ cells compared to controls following treatment with 8-Br-cGMP (8-Br). This effect was abolished by CNG channel and PKG blockers at D3, D6 and D10. Statistical significance was assessed by ANOVA (F_3,76=8.25, <i>P</i><0.0001, at D3; F_3,85=38.5, <i>P</i><0.0001, at D6; F_3,87=32.8, <i>P</i><0.0001, at D10). The Dunnett’s post-hoc test was used for multiple comparisons: #<i>P</i><0.05 and # #<i>P</i><0.001 vs. control; *<i>P</i><0.05 and **<i>P</i><0.001 vs. 8-Br-cGMP-treated NSCs. (<b>B</b>–<b>D</b>) Representative images of MAP2⁺ NSCs at D6 in control differentiation medium (<b>B</b>) and after exposure to 1 mM 8-Br-cGMP (<b>C</b>) or 8-Br-cGMP plus 50 µM LCD (<b>D</b>). Scale bar: 75 µm.