CNG channel-like conductance in differentiating hippocampal NSCs.

<p>Representative whole-cell patch-clamp recordings from NSCs grown for three days in differentiation medium and exhibiting morphological features of neurons. Application of 8-Br-cGMP (1 mM) elicited inward currents at holding potential of -80 mV (<b>A</b>) which were inhibited by the CNG channel blockers, 50 µM LCD (<b>B</b>) and 3 mM Cd²⁺ (<b>C</b>). (<b>D</b>) Voltage ramp protocol used to evoke leakage currents. (<b>E</b>) Typical current traces under control conditions and in the presence of 1 mM 8-Br-cGMP. 8-Br-cGMP-activated current (net current) was obtained by subtraction. (<b>F</b>) I–V plot of mean 8-Br-cGMP net currents (<i>n</i>=10).