The relative gene expression of <i>OCT4</i>, <i>SOX2</i> and the neuronal marker <i>TH</i> mRNA under different growth conditions.

<p>Cells grown as neurospheres in GM (red bar, N/GM), cells grown as neurospheres in DM (blue bar, N/DM), cells grown as monolayer in GM (green bar, A/GM), and cells grown as monolayer in DM (black bar, A/DM) for 5 days. Expression was normalized to GAPDH mRNA expression. Results are expressed as mean ± S.E.M. as a percentage of the highest value in the group set to 100%. n = 5 *p<0.05, ****p<0.0001 neurosphere in GM compared to monolayer cell growth in GM, +p<0.05 +++p<0.001 neurospheres in DM compared to monolayer cells cultured in DM.