Plots showing mRNA expression of various developmental markers for all three iPS lines at each stage ofoligodendrocyte differentiation, as measured using qRT-PCR and normalized to fetal fibroblast controls.

<p>Expression of pluripotency associated genes (A) <i>NANOG</i>, (B) <i>Octamer-binding transcription factor 4 (OCT4)</i> also known as <i>POU5F1 (POU domain</i>, <i>class 5</i>, <i>transcription factor 1)</i> and (C) <i>SRY (sex determining region Y)-box 2 (SOX2) were undetected in oligodendrocyte progenitors (OPs)</i> whilemarkers indicative of the oligodendrocyte lineage were induced including (D) <i>NG2</i>, (E) <i>Myelin-associated glycoprotein</i> (<i>MAG)</i>, and (F) <i>Myelin-associated oligodendrocytic basic protein</i> (<i>MOBP)</i>. Delta CTs were calculated using β-actin as a relative standard and lines normalized to fetal fibroblast controls. Mean <u>+</u> SEM were calculated from two independent differentiation experiments for each line and each gene performed in technical triplicate.Positive control included total RNA from human fetal brain. Abbreviations are Undiff: Undifferentiated iPS cells, EB: embryoid body, NP: neural progenitor, GP: glial progenitor, OP: oligodendrocyte progenitor.