EGFP expression is detected in ECs via flow cytometry.

<p>(A) Representative dot plot of ECs isolated from spleens of transgenic mice. The EC population was selected based on size and labelling with PECAM-1. (B) The majority of splenic ECs express EGFP and PECAM-1. (C) Western blot of brains homogenates (H), as well as soluble (S1) and insoluble (S2) fractions from lysates of ECs isolated from CNS vessels of transgenic animals and immunoblotted for EGFP and p120. <i>n</i>=3 animals.