EGFP expression is not restricted to central nervous system, but is also expressed in vascularized tissues.

<p>(A) Schematic of p<i>Hb9</i>-MitoEGFP transgenic construct, with mitochondrial targeting sequence of Cytochrome <i>c</i> Oxidase subunit VIII. (B) RT-PCR of EGFP mRNA from a panel of tissues of transgenic (+) and non-transgenic littermates (-). Actin serves as a loading control. (C) EGFP protein levels detected via immunoblotting in a panel of tissues isolated from transgenic (+) and non-transgenic littermates (-). SOD1 serves as a loading control. <i>n</i>=3-4 animals.