eGFP transgene expression is comparable between IP and RP preparation methods.

<p>Liver tissue was obtained from 129/S mice injected intraperitoneally with PBS, IP-rAAV8-CMV-eGFP, or RP-rAAV8-CMV-eGFP. Indicated doses for rAAV8 vectors are 0.75 x 10¹¹ vg/mouse (Low), 2.25 x 10¹¹ vg/mouse (Mid), and 7.50 x 10¹¹ vg/mouse (High). <b>(A)</b> Liver tissue lysate was analyzed by anti-GFP immunoblotting. Anti-<i>β</i> actin immunoblotting is shown as a loading control. <b>(B)</b> Isolated liver RNA was analyzed by quantitative RT-PCR for eGFP transcript level. Expression is represented as relative to the 0.75 x 10¹¹ vg RP-rAAV8-CMV-eGFP data set (<i>β</i> actin-normalized). Data are represented as scatter plot with mean ± S.E.M. bars (n = 3 to 6 mice). Unpaired t-tests with Welch’s correction were performed to determine p-values.