The responsiveness of the <i>miR-302</i> promoter to Wnt/β-catenin signaling depends on Tcf BS 5 and Tcf BS 6 and the Oct4/Nanog BS.

<p>Luciferase assays were carried out to examine the activities of different pGL4.10-miR-302 promoter constructs upon β-catenin knockdown (A) or Wnt3a treatment (B,C) (Peprotech; 100 ng/ml). Luciferase activity was normalized to the solvent control (BSA/PBS) for each construct. A, mESCs were transfected with β-catenin specific siRNAs. Twenty-four hours after the transfection of siRNAs, the indicated plasmids were transfected and luciferase assays were performed 24 hours later. B,C, mESCS (B) or P19 cells (C) were transfected with the indicated plasmids and treated with Wnt3a. Twenty-four hours later, luciferase assays were performed. Results of three (A,B) or two (C) independent experiments are shown. Error bars indicate standard error of the mean (SEM). Statistical significance is indicated; Student’s t test *<i>p</i><0.05, **<i>p</i><0.01, ***<i>p</i><0.001, ns: not significant. BS: binding site.