miR-346 promotes activation of the Wnt/β-catenin pathway.

<p>(A) miR-346 overexpression enhanced the expression of both total β-catenin and activated β-catenin (ABC) in hBMSCs. (B) miR-346-overexpressed cells showed more β-catenin accumulation in the nuclei compared with the control cells. (C) Luciferase activity of TOPFlash/FOPFlash in miR-346-overexpressing cells. (D) Western blotting analyses of β-catenin expression in hBMSCs infected with miR-346 or β-catenin siRNA. (E) β-catenin deletion almost completely blocked the positive effect of miR-346 on ALP activity. (F) Western blotting analyses of Wnt/β-catenin downstream molecules in miR-346-overexpressing hBMSCs. All data represent mean ± SD of 3 independent experiments with cells from 3 different donors. **<i>p</i><0.01.