<p>Average expression consistency (M) was calculated with the GeNorm algorithm <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0072884#pone.0072884-Vandesompele1" target="_blank">[18]</a> based on qRT-PCR for the indicated control gene on a panel of 14 leukemia samples and one cord blood sample. Lower M values relate to genes which proved to have more consistent expression levels across the samples used.</p
The measurement of transcript levels constitutes the foundation of today’s molecular genetics. Indep...
<p>(A) Average expression stability values (M) following stepwise exclusion of the least stable refe...
<p>A) Expression stability measurements (M) for the five reference genes analyzed. The x-axis from l...
<p>(A) and (B): Average gene expression stability values M of the remaining reference genes during s...
<p>Expression levels of candidate reference genes in the different tissues by qRT-PCR (A); Average e...
<p>The minimal number of genes required for data normalization was evaluated by pairwise variation (...
<p>(A) Ranking of candidate reference genes according to average expression stability (M value). The...
Background: Measuring messenger RNA (mRNA) levels using the reverse transcription quantitative polym...
<p>Gene expression was measured in blood cell RNA using qRT-PCR to validate the microarray measureme...
<p>For the target genes, the real-time qRT-PCR derived relative expression levels (A, D and G for OP...
Gene expression analysis by quantitative reverse transcription PCR (qRT-PCR) allows accurate quantif...
<div><p>Background</p><p>Measuring messenger RNA (mRNA) levels using the reverse transcription quant...
<p>The letter “A” and “B” in the RNA-Seq and qRT-PCR mean the comparison of expression profiles of s...
<p>qPCR gene expression analysis was carried out on cDNA derived from differentiating cells. Gene ex...
<p>Average pairwise variations (V) were calculated between the normalization factors NFn and NFn+1 b...
The measurement of transcript levels constitutes the foundation of today’s molecular genetics. Indep...
<p>(A) Average expression stability values (M) following stepwise exclusion of the least stable refe...
<p>A) Expression stability measurements (M) for the five reference genes analyzed. The x-axis from l...
<p>(A) and (B): Average gene expression stability values M of the remaining reference genes during s...
<p>Expression levels of candidate reference genes in the different tissues by qRT-PCR (A); Average e...
<p>The minimal number of genes required for data normalization was evaluated by pairwise variation (...
<p>(A) Ranking of candidate reference genes according to average expression stability (M value). The...
Background: Measuring messenger RNA (mRNA) levels using the reverse transcription quantitative polym...
<p>Gene expression was measured in blood cell RNA using qRT-PCR to validate the microarray measureme...
<p>For the target genes, the real-time qRT-PCR derived relative expression levels (A, D and G for OP...
Gene expression analysis by quantitative reverse transcription PCR (qRT-PCR) allows accurate quantif...
<div><p>Background</p><p>Measuring messenger RNA (mRNA) levels using the reverse transcription quant...
<p>The letter “A” and “B” in the RNA-Seq and qRT-PCR mean the comparison of expression profiles of s...
<p>qPCR gene expression analysis was carried out on cDNA derived from differentiating cells. Gene ex...
<p>Average pairwise variations (V) were calculated between the normalization factors NFn and NFn+1 b...
The measurement of transcript levels constitutes the foundation of today’s molecular genetics. Indep...
<p>(A) Average expression stability values (M) following stepwise exclusion of the least stable refe...
<p>A) Expression stability measurements (M) for the five reference genes analyzed. The x-axis from l...