GeNorm evaluation of the minimal number of genes and validation of the identified top-ranked HKG genes for qRT-PCR normalization.

<p>The minimal number of genes required for data normalization was evaluated by pairwise variation (Vn/n +1) in (A) CSC, (B) native cells, (C) in the CSC and native cell lines from all tumors, (D) from sarcoma and (C) from carcinomas. A variation coefficient (V) below 0.15 indicates the optimal number of genes required for data normalization. V2/3<0.15 indicates that 2 genes are required for data normalization.