The geNorm analysis for finding the optimal number of finger millet reference genes essential for precise and accurate normalization studies in all four sample sets.
- Palakolanu Sudhakar Reddy (530583)
- Mahamaya G. Dhaware (5856095)
- Dumbala Srinivas Reddy (475340)
- Bommineni Pradeep Reddy (5856098)
- Kummari Divya (5856101)
- Kiran K. Sharma (475343)
- Pooja Bhatnagar-Mathur (475341)
Publication date
October 2018
DOI Publisher
Public Library of Science (PLoS)Abstract
The pairwise variation (Vn/Vn+1) was analyzed by the geNorm statistical tool to decide the minimum number of reference genes for precise and accurate RT-qPCR data normalization in each experimental set of finger millet samples. The cutoff value was 0.15; below this value indicates no additional reference gene required for RT-qPCR data normalization.</p
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