<p>A. day 7 and 14 differentiated IPCs were analysed RT-PCR (M: marker; l:β-actin, 2:PDX-1, 3:g1ut2, 4:Ngn3, 5:Isl1, 6:Nkx6.1, 7:PC1, 8:PC2, 9:Sst); B. Quantitative real-time PCR analyses for the expressions of PDX-1, g1ut2, Ngn3, Isl1,Nkx6.1,PC1, PC2 and Sst. Results are the means ± SEMs for 3 times.</p
<p>A: Semi-quantitative RT PCR comparison between RYAS41, lung (negative control) and pancreas (posi...
<p><b>A</b>. Hormone gene expression in single cells. Every three lanes on the gel contain RT-PCR pr...
Background: The last decade has seen significant progress in understanding the molecular biology of ...
<p>(A) RT-PCR analysis for mRNA expression of pancreatic β-cell markers in differentiated iTS-P cell...
Additional file 3: Table S3. List of primers used for quantitative real-time PCR reactions
<p>The expression of each gene is illustrated as expression of SYBR green (semi-log scale in the Y-a...
Additional file 8: Table S7. Comparison of relative SOX17, FOXA2 and CXCR4 expression levels in defi...
<p>(A) Exact copy number of insulin mRNA molecules in the ES-DBCs and human islets by digital drople...
<p>Cells were induced to differentiate through-out the whole protocol as indicated in Fig. 1 and ana...
<p>(A) Phase contrast images of wild-type (WT) mESCs and menin-null (KO) mESCs differentiated into P...
<p>Western blot analysis detected expressions of insulin (5KD), glucagon (46KD), PDX1 (31KD) and Ngn...
<p>A) Cells were induced to differentiate through progression of stages 1 and 2 with or without DM a...
<p>The cells were cultured as EBs in different concentrations of MPE (mouse pancreas extract). (A) R...
SR1423 differentiation yields robust pancreatic, hormone-secreting cell populations. (A) SR1423 diff...
<p>In A, immunocytochemical detection of nestin, glucagon and insulin in stage 2 and stage 4 of diff...
<p>A: Semi-quantitative RT PCR comparison between RYAS41, lung (negative control) and pancreas (posi...
<p><b>A</b>. Hormone gene expression in single cells. Every three lanes on the gel contain RT-PCR pr...
Background: The last decade has seen significant progress in understanding the molecular biology of ...
<p>(A) RT-PCR analysis for mRNA expression of pancreatic β-cell markers in differentiated iTS-P cell...
Additional file 3: Table S3. List of primers used for quantitative real-time PCR reactions
<p>The expression of each gene is illustrated as expression of SYBR green (semi-log scale in the Y-a...
Additional file 8: Table S7. Comparison of relative SOX17, FOXA2 and CXCR4 expression levels in defi...
<p>(A) Exact copy number of insulin mRNA molecules in the ES-DBCs and human islets by digital drople...
<p>Cells were induced to differentiate through-out the whole protocol as indicated in Fig. 1 and ana...
<p>(A) Phase contrast images of wild-type (WT) mESCs and menin-null (KO) mESCs differentiated into P...
<p>Western blot analysis detected expressions of insulin (5KD), glucagon (46KD), PDX1 (31KD) and Ngn...
<p>A) Cells were induced to differentiate through progression of stages 1 and 2 with or without DM a...
<p>The cells were cultured as EBs in different concentrations of MPE (mouse pancreas extract). (A) R...
SR1423 differentiation yields robust pancreatic, hormone-secreting cell populations. (A) SR1423 diff...
<p>In A, immunocytochemical detection of nestin, glucagon and insulin in stage 2 and stage 4 of diff...
<p>A: Semi-quantitative RT PCR comparison between RYAS41, lung (negative control) and pancreas (posi...
<p><b>A</b>. Hormone gene expression in single cells. Every three lanes on the gel contain RT-PCR pr...
Background: The last decade has seen significant progress in understanding the molecular biology of ...