Identification of cell-types in rat pancreatic islets.

<p><b>A</b>. Hormone gene expression in single cells. Every three lanes on the gel contain RT-PCR products amplified with insulin (I), glucagon (G) and somatostatin (S) primers: Ins (195 bp), Gcg2 (328 bp) and Sst2 (140 bp), respectively (from left to right). The cell-type was identified according to which product had been detected. Positive controls were purified rat islets RNA samples and the negative control was water. <b>B</b>. The pie diagram illustrates the proportion of different cell-types discriminated by single-cell RT-PCR. <b>C.</b> Cell capacitance of α-cells (n = 18) and β-cells (n = 12). *P<0.05.