MEIOB is located at the DNA double strand breaks.

<p>(<b>A</b>) SYCP3, MEIOB and RPA2, RAD51 or DMC1 were detected in chromosome spreads of zygotene wild type spermatocytes. RPA2 and MEIOB were mostly colocalized at these stages. White squares indicate magnified regions. (<b>B</b>) MEIOB staining in <i>Spo11^−/−</i> spermatocytes that were defective in DSB formation. Hardly any foci were observed on the chromosome axes in <i>Spo11^−/−</i> spermatocytes. (<b>C</b>) MEIOB staining in <i>Dmc1^−/−</i> spermatocytes that were arrested due to absence of strand invasion. MEIOB foci in <i>Dmc1^−/−</i> appeared brighter in comparison to <i>Dmc1^+/+</i>.