RPA1 is required for focal localization of RAD51/DMC1 on meiotic chromosomes but not MEIOB/SPATA22 at the zygotene stage.
- Baolu Shi (2328775)
- Jiangyang Xue (4762836)
- Hao Yin (381910)
- Rui Guo (134395)
- Mengcheng Luo (687507)
- Lan Ye (140528)
- Qinghua Shi (169845)
- Xiaoyan Huang (112885)
- Mingxi Liu (129494)
- Jiahao Sha (129501)
- P. Jeremy Wang (119396)
Publication date
February 2019
DOI Publisher
Public Library of Science (PLoS)Abstract
(A) Immunolocalization of ssDNA-binding proteins in zygotene (control) and zygotene-like (Rpa1fl/fl Ddx4-CreERT2, 4 dpt) spermatocytes. Synaptonemal complexes were immunostained with anti-SYCP2 antibody. Note that γH2AX signal is much stronger in mutant zygotene-like spermatocytes than control zygonema. (B) Dot plots of foci of RAD51, DMC1, MEIOB, and SPATA22 in zygotene (control) and zygotene-like (Rpa1cKO) spermatocytes. Solid lines show the average ± SD. n, number of cells counted from two experiments; ***, p t test); ns, non-significant. (C) A role for RPA in pre-synaptic filament formation. Upper panel, control; bottom panel, absence of RPA.</p
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