Immunoblot analysis of TP and A160T using the monoclonal FLAG antibody.

<p>TP and the A160T variant expressed in HEK293S-TetR stable cell line (lanes A and C). By using a HEK293S (GnTI^-) cell line defective in N-acetylglucosamine transferase I, TP and A160T were expressed with restricted and homogeneous N-glycosylation (lanes B and D). 5 µl of protein were loaded into all wells, and western blot analysis carried out using the FLAG antibody. Mobility of molecular weight standards is indicated next to the gel. The epitope tag for the monoclonal FLAG antibody was added to the N-terminus of the TP receptor.