FBG1 immunoprecipitates normal and mutant versions of A1AT.

<p>Upper panel: the input came from the total cell lysate of Hepa1-6 cells co-transfected with pCMV or FBG1, and the indicated mixes of A1AT variants. FLAG co-immunoprecipitation (IP) lanes were probed with anti-A1AT that recognizes the M, Z, and K forms of A1AT. Middle panel: anti-FLAG antibody detects FLAG-FBG1, indicating successful transfection of FLAG-FBG1 in cells. Bottom panel: total protein indicates roughly equal loading of protein. The lack of total protein staining in the IP lanes is expected, as the amount of protein pulled down in an IP is below detection limits.