N-glycosylation increases the presence of cell surface isoforms of GIPR and GLP-1R protein.

<p>Western Blots of biotinylated cell surface protein (upper) and total protein lysate (middle) for V5-tagged GIPR (left) and GLP-1R (right). Upper and middle blots were probed with a mouse monoclonal antibody to the V5 epitope while the lower blots were probed with a rabbit polyclonal antibody to actin as a loading control. Note the absence of biotinylated protein in the lane containing N62,77Q-GIPR but not in the lane containing N63,82,115Q-GLP-1R.