RUNX2 17A and 11A constructs have differential nuclear residence.

<p>A. Confocal immuno-fluorescence labeled with anti-RUNX2 antibody to detect nuclear or cytoplasmic residence. A comparison of RUNX2 17A and 11A reveals small differences in RUNX2 nuclear residence. B. the ability of RUNX2 to enhance the nuclear localization of the partner protein CBFB is diminished in RUNX2 11A isoform. White numbers indicate percentages of cells with that particular type of staining. C. Western blot indicating that expression vectors produce appropriate amounts of RUNX2 variants. Left and right panels are derived from the same gel, with intervening lanes removed; the left panel with mock transfected control and wild type 17A control appeared previously <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0072740#pone.0072740-Morrison1" target="_blank">[24]</a>.