<p>Immunolocalizations of FLAG-tagged wild-type, T174I, and L205R MSX1 products were similar when these variants were expressed in HEK293 cells. MSX1 (FLAG, red signal); nuclei (DAPI, blue signal). Cell morphologies were visualized by F-actin staining (Phalloidin, green signal). Neither the T174I nor L205R mutation affects the nuclear localization of MSX1 in vitro. Scale bar 20 µm.</p
<p>HeLa cells were transfected with GFP vector (first row) wild-type A2-GFP (second row) or A2<sup>R...
<p>(A) Co-immunoprecipitation assays were done using C2C12 cell protein extracts expressing Flag-Msx...
<p>HEK293T cells were transiently transfected with empty vector (Mock), wild-type (WT) or mutant (MT...
<p>(A) Immunolocalization of FLAG-tagged MSX1 protein in transfected COS7 cells. Nuclear translocati...
MSX1 is one of the homeoproteins with the homeodomain (HD) sequence, which regulates proliferation a...
MSX1 is one of the homeoproteins with the homeodomain (HD) sequence, which regulates proliferation a...
<p>MAML1 was mutated at various lysine residues as described in text. Myc-tagged MAML1 Wild type (WT...
<p>(A) ChIP-qPCR analyses of H3K27me3 mark on Msx1 target genes in C2C12 cells lacking Msx1 or expre...
<p>A: Immunofluorescent detection of endogenous Ms1 in NRCs. Staining is (left-to-right) DAPI (nucle...
<p>Both wild-type and mutant proteins display nuclear localization, as evidenced by co-localization ...
<p>(A) The positions of the putative NLS motifs and their amino acid sequences are highlighted in th...
<p>MC3T3-E1 cells were transfected with WT or mutated FLAG-tagged β-catenin constructs, treated with...
<p>LHCN-M2 cells were transfected with a DUX4c or DUX4 expression vector (<i>p-DUX4</i>, <i>p-DUX4c<...
<p>(A) F-IHC analysis of 293T cells transiently transfected with MSCV-CDX2-Flag or MSCV-CDX2/AS-His ...
<p>A.) U2OS osteosarcoma cells were transfected with wt RPS9-FLAG and RPS9-FLAG deletion mutants as ...
<p>HeLa cells were transfected with GFP vector (first row) wild-type A2-GFP (second row) or A2<sup>R...
<p>(A) Co-immunoprecipitation assays were done using C2C12 cell protein extracts expressing Flag-Msx...
<p>HEK293T cells were transiently transfected with empty vector (Mock), wild-type (WT) or mutant (MT...
<p>(A) Immunolocalization of FLAG-tagged MSX1 protein in transfected COS7 cells. Nuclear translocati...
MSX1 is one of the homeoproteins with the homeodomain (HD) sequence, which regulates proliferation a...
MSX1 is one of the homeoproteins with the homeodomain (HD) sequence, which regulates proliferation a...
<p>MAML1 was mutated at various lysine residues as described in text. Myc-tagged MAML1 Wild type (WT...
<p>(A) ChIP-qPCR analyses of H3K27me3 mark on Msx1 target genes in C2C12 cells lacking Msx1 or expre...
<p>A: Immunofluorescent detection of endogenous Ms1 in NRCs. Staining is (left-to-right) DAPI (nucle...
<p>Both wild-type and mutant proteins display nuclear localization, as evidenced by co-localization ...
<p>(A) The positions of the putative NLS motifs and their amino acid sequences are highlighted in th...
<p>MC3T3-E1 cells were transfected with WT or mutated FLAG-tagged β-catenin constructs, treated with...
<p>LHCN-M2 cells were transfected with a DUX4c or DUX4 expression vector (<i>p-DUX4</i>, <i>p-DUX4c<...
<p>(A) F-IHC analysis of 293T cells transiently transfected with MSCV-CDX2-Flag or MSCV-CDX2/AS-His ...
<p>A.) U2OS osteosarcoma cells were transfected with wt RPS9-FLAG and RPS9-FLAG deletion mutants as ...
<p>HeLa cells were transfected with GFP vector (first row) wild-type A2-GFP (second row) or A2<sup>R...
<p>(A) Co-immunoprecipitation assays were done using C2C12 cell protein extracts expressing Flag-Msx...
<p>HEK293T cells were transiently transfected with empty vector (Mock), wild-type (WT) or mutant (MT...