Runx2-I was expressed in <i>Runx2-I^TGA/TGA</i> mice as N-terminal truncated Runx2.

<p>(A) Anti-Runx2 mAb (clone: 8G5) could recognize Runx2-II, Runx2-I, and their truncated versions. HEK293 cells were transiently transfected with the indicated expression vectors (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0108294#s2" target="_blank">Materials and Methods</a>). Forty-eight hours after transfection, nuclear proteins were obtained from the cells and then immunoblotted with anti-Runx2 mAb. (B) Nuclear proteins of calvarial cells freshly prepared from embryos (18.5 dpc) with the genotypes indicated in the figure were immunoblotted with anti-Runx2. The membranes were also blotted with anti-Lamin A/C mAb as an internal control. The data are representative of three independent experiments.