EXLX1 production, purification and filter paper disruption.

<p>(a) Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis: Lane M, protein molecular weight marker; Lane 1, the total cell lysate of the culture without IPTG; Lane 2, the soluble fraction of the culture without IPTG; Lane 3, the total cell lysate of the culture with IPTG; Lanes 4, the soluble fraction of the culture with IPTG; Lane 5, purified EXLX1 (about 26 kDa calculated based on amino acid sequences). (b) Light microscopy graphs of filter paper incubated with the soluble fraction of IPTG-induced culture, soluble fraction of the culture without IPTG and buffer A at 30°C for 12 h, respectively. Both the cell lysate and the soluble fraction were dissolved in buffer A (50 mM NaH₂PO₄, 500 mM NaCl, pH 8.0).