Purification scheme and analysis of BG505 SOSIP.664 trimers in ExpiCHO-S cultures.
- Albert Cupo (409522)
- Victor M. Cruz Portillo (4839432)
- Paul Gelfand (6564815)
- Anila Yasmeen (460713)
- P. J. Klasse (338325)
- John P. Moore (260880)
Publication date
April 2019
DOI Publisher
Public Library of Science (PLoS)Abstract
(A) The culture supernatant (red flask) was processed via the affinity columns, in the manners indicated by the arrows, leading to the purified trimer yields recorded below each column. (B) Coomassie blue-stained BN-PAGE analysis of ExpiCHO-S expressed BG505 SOSIP.664 trimer purified by the different affinity columns. The molecular masses of marker (M) proteins (thyroglobulin and ferritin) are indicated.</p
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