FISH on extended genomic DNA fibers and BAC molecules.

<p>A) FISH signals derived from two BAC clones 173C03 (green) and 174A01 (red) on extended genomic fibers of <i>G. hirsutum</i>. B) FISH mapping of BAC 173C03 molecules. Both clones 174A01 (red) and BAC 173C03 (green) were used as probe and hybridized onto 173C03 molecules to visualize the BAC 173C03 molecules and overlap regions. Red signals (174A01) covered most parts of BAC 173C03 molecules indicating that most parts of 173C03 were overlapped with 174A01. C) FISH mapping of BAC 174A01 molecules. Probes 174A01 (green) and BAC 173C03 (red) were hybridized onto 174A01 molecules. To facilitate the identification of overlapped regions, BAC vector (red, arrow) was labeled and hybridized simultaneously with two BAC probes. Bars are 10 µm.