Examples of BAC-FISH mapping of Z-linked genes on the <i>Cydia pomonella</i> neo-Z chromosome.

<p>Cy3-dUtP-labeled (red hybridization signals) and Fluorescein-12-dUTP-labeled (green) BAC probes were used for BAC-FISH mapping in spread chromosome preparation from testes, counterstained with DAPI (blue). A: CpS-Krym mitotic spermatogonial metaphase showing 2n = 56 chromosomes; two neo-Z chromosomes marked with hybridization signals of BAC probes containing <i>per</i> (green) and <i>nan</i> (red) genes. B: Detailed view of pachytene ZZ bivalent of CpRR1 with hybridized <i>ap</i> (terminal red, arrow), <i>Tpi</i> (green) and <i>Idh-2</i> (interstitial red) probes. C: CpR5M pachytene complement showing a long ZZ bivalent with hybridized <i>ap</i> (terminal red), <i>ABCF2</i> (green) and <i>Notch</i> (interstitial red, arrow) probes.