Intranasal administration of clodronate-liposomes selectively depletes CD11c⁺F4/80⁺alveolar macrophages in lungs and bronchoalveolar lavages.

<p>The cells from the bronchoalveolar lavage (BAL) and lungs from mice (n = 3) were harvested4 days after clodronate injection. CD3⁺ and CD11b⁺ cells were excluded to determine the effect of clodronate on depleting alveolar macrophages with CD3⁻CD11b⁻CD11c⁺F4/80⁺markers. (A) Representative profiles of flow cytometry gating of CD3⁻CD11b⁻CD11c⁺F4/80⁺phenotypic cells. (B) Percentages of CD3⁻CD11b⁻CD11c⁺F4/80⁺cells. (C) Cellularity of CD3⁻CD11b⁻CD11c⁺F4/80⁺cells. (−) Clodronate or (−)Clod: No clodronate control mice, (+) Clodronate or (+)Clod: Clodronate treated mice. BAL: Bronchoalveolar cells, Lung: Cells collected from lung tissues. *P<0.05, **P<0.001, ***P<0.0001 (Student T-test). Bars indicate standard errors.