Intraperitoneal injection of clodronate-containing liposomes depletes macrophages in visceral white adipose tissue and liver in mice.

<p>A: the total number of crown-like structures/10 visual fields and the number of F4/80 positive cells/field in liver. Asterisks denote significant differences from control. B: Representative images of visceral white adipose tissues from control (a) and clodronate-containing liposome-treated mice 5 (b) and 14 days (c) after injections. F4/80 positive macrophages around adipocytes (crown-like structures) are indicated by asterisks. Representative images of liver from control (d) and clodronate-containing liposome-treated mice 5 (e) and 14 days (f) after injections.