Detection of brazzein in the milk of transgenic mice.

<p>(A) Expression of brazzein in the mammary glands of transgenic mice under western blot analysis. Skim milk samples (1 uL each) were separated by SDS-PAGE and immunoblotted with anti-brazzein antibodies; WT, non-transgenic milk; 1, 2, and 3, milk from transgenic mice; 3-F2a to 3-F2e represent the milk produced by the F2 generation of mouse line (B) Immunofluorescent histochemical stains of brazzein in two sections of the mammary gland tissues. Non-transgenic mammary gland tissues were used as the negative control. Scale bars = 50 um. (C) Transgenic mouse mammary gland tissues. Scale bars = 50 um. (D) Hematoxylin and eosin staining of transgenic mammary gland tissues. Scale bars = 50 um. (E) Brazzein concentrations in the milk of different mice. Control, the milk of a non-transgenic mouse; 1, 2, 3, 3-F2a, 3-F2b, 3-F2c, 3-F2d, and 3-F2e are milk samples from the different transgenic mice.