RT-PCR analysis of transgenic mouse from various tissues to determine relative transcripts levels of brazzein.

<p>(A) RT-PCR was performed to determine the tissue specificity and expression levels of brazzein in transgenic mice. The tissues analyzed were from liver (li), heart (ht), spleen (sp), lungs (lu), kidneys (ki), pancreas (pa), uterus (ut), and mammary glands (mg). M represents Marker; -RT represents no M-MuLV Reverse Transcriptase in the reaction to rule out genomic DNA contamination. The bottom panel is the result of the RT-PCR analysis of the mouse GAPDH gene. (B) The relative transcripts of brazzein in mammary gland tissues detected by real-time PCR were much higher than in other tissues. Total mRNA was extracted from mammary tissues of mouse lines 1, 2, and 3.The results are shown as means ± S.D. ***P<0.001 compared with control.