Mammary gland expression of the bFcRn transgenes in transgenic mice.

<p>(A) <i>bFcRn α</i> mRNA levels in the mammary gland of transgenic mice. The expression levels were determined by the expression related to <i>GAPDH</i> (an internal control). The data were combined from three independent experiments; the bars represent the means ± SD (n = 7–8 mice per group); Neg, wild-type mice as a control; <i>**P</i><0.01. (B) Western blot of bFcRn in the four lines of transgenic mice. HRP-conjugated K6 antibody against a polypeptide (AGLAQPLTVE) representing amino acids 279–288 was used. Tubulin was used as a loading control. Neg-41, protein from a wild-type mouse as a control; P, bovine mammary gland. (C) Immunofluorescent analysis of the mammary gland during lactation. Tissue sections prepared from the mammary gland were stained with the FITC-conjugated anti-bFcRn α antibody (K6) (Green). DAPI (blue) indicated nuclear staining. The data are representative of at least three sections. Images were obtained with a 40X water objective lens.