miR-34a and DGKζ mRNA expression in naïve and effector T cells.

<p>(<b>A</b>) Conserved seed matches on DGKζ mRNA are designated with the gray box. (<b>B</b> and <b>C</b>) Naïve T cells sorted from wild-type mice were incubated with anti-CD3ε and anti-CD28 antibodies for 48 hours. mRNAs from naïve and activated CD4⁺ and CD8⁺ T cells were reversely transcribed. miR-34a and DGKζ levels were quantified by real-time qPCR. Bar graphs represent mean ± SEM from three independent experiments (a.u., arbitrary unit; ***<i>P</i> < 0.001).