Effect of UPEC infection on <i>Defb1</i> mRNA expression.

<p>(A) Timecourse of <i>Defb1</i> mRNA expression in bladders (open boxes) versus kidneys (shaded boxes) of UPEC infected mice. Time in hpi is indicated on the X axis. Samples were normalized for <i>Gapdh</i> mRNA expression and compared to a pool of uninfected bladder or kidney cDNA using the 2^-ΔΔCT method[<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0077714#B22" target="_blank">22</a>]. Means ± S.E.M. are shown (n=4 bladders and 4 kidneys/group) * <i>p</i> < 0.05, student’s <i>t</i>-test, indicated timepoint versus uninfected bladders; # p < 0.05, student’s t-test, indicated timepoint versus uninfected kidneys. (B) <i>Defb1</i> mRNA was measured in ureters from uninfected C57BL/6 females (shaded box) and 16 hpi with UTI89 (open box). Samples were normalized for <i>Gapdh</i> content and expressed as fold-difference compared to a pool of uninfected mouse ureter cDNA using the 2^-ΔΔCT method. There was no significant difference in <i>Defb1</i> expression between groups. (C) Bladder <i>Defb1</i> mRNA expression is down-regulated in C3H/HeN mice 16 hpi, but kidney <i>Defb1</i> mRNA levels are unchanged. Shaded boxes represent uninfected organs, and open boxes represent organs harvested 16 hpi. Samples were normalized for <i>Gapdh</i> mRNA expression and compared to a pool of uninfected kidney cDNA using the 2^-ΔΔCT method[<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0077714#B22" target="_blank">22</a>]. Means ± S.E.M. are shown (n=3 bladders and 3 kidneys/group) * <i>p</i> < 0.05, student’s <i>t</i>-test, naïve versus infected C3H/HeN bladders.