Macrophage depletion does not impact effector cell infiltration or cytokine expression.

<p>(A-B) Female C57Bl/6 mice were infected and then challenged with UPEC after resolution of their primary infection, as in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1005044#ppat.1005044.g001" target="_blank">Fig 1A</a>. Twenty-four hours post-challenge, cellular infiltration into the bladder was evaluated by flow cytometry. Graphs depict cell number per bladder of the indicated populations. Experiment was repeated 3 times with 5–7 mice per group. (C) Mice were depleted with anti-CSF1R antibody and infected with 1x10⁷ CFU of UTI89 24 hours after depletion. Mice were sacrificed 24 hours P.I. and bladders were homogenized. Samples were stored at -80°C until all samples could be assessed together by Luminex multi-analyte profiling. Graphs depict the expression levels of selected cytokines in isotype antibody treated (black dots) and depleting-antibody treated (open circles) mice. Analytes are grouped by high expression (left graph) and low or no expression (right graph). Each dot represents a mouse, experiment performed 2 times with 5 mice per group and pooled, lines are medians. Additional analytes are shown in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1005044#ppat.1005044.s006" target="_blank">S6 Fig</a>.